KMID : 0369820110410040205
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Jorunal of Korean Pharmaceutical Sciences 2011 Volume.41 No. 4 p.205 ~ p.210
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Liposome/Tat Complex for Facilitating Genistein Uptake into B16 Melanoma Cells
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Park Young-Mi
Kang Myung-Joo Moon Ki-Young Park Sang-Han Kang Mean-Hyung Choi Young-Wook
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Abstract
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Genistein (GT), a major isoflavone found in soybeans, has a potent antioxidant effect that protects the skin from UV-induced damages and malignant melanoma. In order to enhance the cellular uptake of GT, liposome/Tat complexes were prepared by an electrostatic interaction of anionic liposome (DMPC/DCP, 9:1 in molar ratio) with Tat peptide (0.02 to 0.08 mole), one of the well-known cell penetrating peptide (CPP). As the amount of Tat increased, the size increased but the zeta potential decreased. In vitro release study with dialysis membrane elicited GT release from liposomal preparations in a controlled manner. The addition of Tat increased GT release, especially for the initial period. In the cellular uptake study by incubating B16 melanoma cells with various liposomal preparations containing GT, B16 melanoma cells demonstrated a time-dependent increase of drug accumulation. Compared to the aqueous GT suspension, intracellular uptake was substantially enhanced by anionic liposomal formulation and further increased by the complex formulation. Therefore, liposome/ Tat complex might be a good candidate for facilitating intracellular drug delivery.
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KEYWORD
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Genistein, Liposome/Tat complex, B16 melanoma cell, Drug release, Cellular uptake
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